The present study explores intermediate stages in detergent solubilization of liposomes and Ca2+-ATPase membranes by sodium dodecyl sulfate (SDS) and medium-sized ( approximately C12) nonionic detergents. In all cases detergent partitioning in the membranes precedes cooperative binding and solubiliz …Web

The aggregation behavior of sodium dodecyl sulfate (SDS) was studied in an aqueous solution in the presence of increasing concentrations of selected sodium 1-alkylsulfonates, namely sodium 1-octanesulfonate, sodium 1-decanesulfonate, and sodium 1-dodecanesufonate. ... Furthermore, the solubilization power (SP) of both surfactants …Web

Solubilization of liposomes made up of 1 mM phosphatidyl choline only by sodium dodecyl sulfate as measured by optical density is given in Fig. 1. Here, the dependence of liposome solubilization on SDS concentration for different interaction times is shown. After 15 min. of interaction, about 35% of the liposome has been solubilized by …Web

Drug solubilization increases linearly with the increase of hydrophobic chain length for all studied surfactant types. Addition of 1-3 ethylene oxide (EO) units in the head group of dodecyl sulfate surfactants reduces significantly the solubilization of both studied drugs and decreases linearly the solubilization locus polarity of fenofibrate.Web

The solubilization mechanism as a function of atazanavir concentration was probed using fluorescence spectroscopy. ... Curcumin was purchased from Spectrum Chemical Mfg. Corp. (Gardena, CA). Sodium dodecyl sulfate (SDS), pyrene carboxaldehyde, pyrene, dodecyl pyridinium chloride, deuterium oxide were obtained …Web

This article proposes a new technique for the solubilization of poorly soluble drugs using lauroyl-L-glutamate, which is one of the amino acid detergents, with additional small additives. ... The synergistic effect was not observed by sodium dodecyl sulphate with arginine. The solubilizing system can be applied to other drugs because of the low ...Web

The partition equilibria of sodium dodecyl sulfate (SDS) and lithium dodecyl sulfate between water and bilayer membranes were investigated with isothermal titration calorimetry and spectroscopic methods (light scattering, (31)P-nuclear magnetic resonance) in the temperature range of 28 degrees C to 56 degrees C.Web

Abstract. A spectrophotometric study of the solubilization and aggregation of the Nile red dye (NR) in premicellar and micellar aqueous solutions of sodium dodecyl sulfate (SDS) was carried out. The experiments were conducted both with saturated solutions of NR under conditions of thermodynamic equilibrium of the solution with a dye precipitate ...Web

The solubilization and physicochemical behavior of a coordination complex of nickel, namely [Ni(im) 6]F 2 ·5H 2 O [hexakis(imidazole)nickel(II) fluoride pentahydrate], in aqueous micellar media of anionic surfactants, i.e., sodium dodecyl sulfate (SDS) and sodium stearate (SS), were investigated by using UV–Vis spectroscopy and electrical …Web

Ability of sodium dodecyl sulfate to transiently stabilize a phospholipid molecular layer. Thin Solid Films 2016, 615, 215-220. …Web

Removal of sodium dodecyl sulphate, urea, CHAPS using filter-aided sample preparation (FASP) and zip tips [66–68] still resulted in interferences to liquid chromatography runs and mass spectrometric analysis [68, 69]. The limitation of the utilization of SDC in protein solubilization, which was observed from the study, was the …Web

This is exemplified for the flip-flop of the anionic detergent sodium dodecyl sulfate (SDS) across the membranes of 100-nm-diameter unilamellar vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in aqueous solution (10 mM phosphate buffer, 154 mM NaCl, pH 7.4). If repulsive electrostatic forces are accounted …Web

The findings support a general mechanism of detergent-induced membrane solubilization and expect the framework of correlative biophysical technologies presented here will form a general platform for elucidating the complex kinetics of membrane perturbation induced by a wide variety of surfactants and disrupting agents. Membrane …Web

This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an ionic detergent that dissolves membranes, disrupts protein-nucleic acid interactions, and inactivates …Web

The solubilization of lipid membranes by the anionic detergent sodium dodecyl sulfate (SDS) has found vast utility across the pharmaceutical and biological sectors with far …Web

In proteomics, sodium dodecyl sulfate (SDS) is favored for protein solubilization and mass-based separation (e.g. GELFrEE or SDS PAGE). Numerous SDS depletion techniques are available to purify proteins ahead of mass spectrometry. The effectiveness of the purification has a controlling influence on the success of the analysis.Web

as a protein and lipid solubilization reagent. In general, for solubilization of proteins, SDS should be used at its critical micelle concentration.2 SDS is also ... Sodium Dodecyl Sulfate from Proteins. Anal. Biochem., 91(1), 230-235 (1978). 8. Hayashi, K., A Rapid Determination of Sodium Dodecyl Sulfate with Methylene Blue. Anal.Web

as a protein and lipid solubilization reagent. As a general rule for the solubilization of proteins, SDS should be used at its critical micelle concentration.2 SDS is also a …Web

The apparent dissociation constants of 1-propanoic, 1-butanoic, 1-pentanoic and 1-hexanoic acids were obtained for the first time in Brij 35 micellar solutions with concentration from 0.03 to 0.20 mol⋅L−1 and sodium dodecyl sulfate (SDS) micellar solutions with concentrations from 0.01 to 0.30 mol⋅L−1. A pronounced effect of Brij 35 …Web

The maximum solubilization capacity can be obtained from the intersection of the two straight lines at low and high 1-hexene concentration. Below the maximum solubilization capacity, micelles are still capable of solubilizing some further1-hexene; this phenomenon is called "swollen micelles" according to Kralchevsky's research [33], [34 ...Web

Membrane solubilization by sodium dodecyl sulfate (SDS) is indispensable for many established biotechnological applications, including viral inactivation and protein extraction. Although the ensemble thermodynamics have been thoroughly explored, the underlying molecular dynamics have remained inaccessible, owing to major limitations of ...Web

The process of recovering bioactive proteins from IBs consists of solubilization of IB aggregates using denaturants, followed by refolding of the solubilized protein. ... Chaotropic agents such as urea and GdnHCl, in the presence of low concentration of detergents such as sodium dodecyl sulfate (SDS), sodium …Web

A straight chain alkyl tail seemed to be better than an alkylaryl tail, as judged from the comparison of solubilization power for the pairs sodium dodecyl sulphate (SDS)/sodium dodecyl benzene ...Web

SDS has a wide variety of applications, but is most often used as a protein and lipid solubilization reagent. As a general rule for the solubilization of proteins, SDS should …Web

The partition equilibria of sodium dodecyl sulfate (SDS) and lithium dodecyl sulfate between water and bilayer membranes were investigated with isothermal titration calorimetry and spectroscopic methods (light scattering, 31P-nuclear magnetic resonance) in the temperature range of 28°C to 56°C. The partitioning of the dodecyl sulfate anion (DS−) …Web

INTRODUCTION. This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an …Web

The interaction of mixtures of sodium dodecyl sulfate (SDS) and oxyethylenated nonylphenol (30 mol of ethylene oxide) [NP(EO) 30] with phosphatidylcholine liposomes was investigated.Permeability alterations were detected as a change in 5(6)-carboxyfluorescein (CF) released from the interior of vesicles, and bilayer solubilization was measured as a …Web

We provide a comprehensive thermodynamic description of lipid membrane dissolution by a charged detergent. To this end, we have studied the interactions between the anionic detergent sodium dodecyl sulfate (SDS) and the zwitterionic phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in dilute aqueous solution (10 …Web

In order to develop surfactant-enhanced remediation for nitrogen heterocyclic compounds (NHCs) (aniline, indole, and quinolone), the solubilization properties of micellar solutions of five surfactants, namely sodium dodecyl sulfate (SDS), rhamnolipid (RL), polysorbate (Tween 80), sorbitan monolaurate (Span 20), and iso-octyl phenoxy …Web

The interaction between sodium dodecyl sulfate (SDS) and a phospholipid (1,2-dioleoyl-sn-glycero-3-phosphocholine ... In contrast, biomembranes are damaged by the penetration of surfactant molecules in the lipid bilayers or the solubilization of lipid molecules in surfactant micelles [3], [4], [5]. On the other hand, the surfactants are ...Web

Precise conductivity measurements of sodium dodecyl sulfate (SDS) solutions with and without manganese sulfate in water and ethanol–water mixture containing (10, 20 and 30) volume % ethanol is carried out at 298.15 K. ... The decrease in α of SDS in presence of alcohol can be attributed to the solubilization of alcohol in the palisade layer ...Web